It should give you all of that information. It’s been awhile since I’ve used it, but it’s not really that complicated. You can use different searches to search different databases (nucleotide sequence vs. database, nucleotide sequence against protein database, etc.)

Enter the sequence using FASTA format. Basically.

>Sequence Name (you can enter testsequence, seq1, etc)

DNA sequence (GCAAGTCA, etc.)

If you’re still having problems post the sequence and I might be able to take a look at it.

P.S. I looked at the site listed above. It’s kind of a cool little program. But it’s not quite what you need. It’s more for finding restriction sites in a sequence.

http://www.acaclone.com/

If that is not what you need, I just typed in dna analysis in the search box…you might want to add “gene” before analysis. Good luck!