A few days ago
Anonymous

Paper Chromatography! Two urgent questions!?

In paper chromatography, why is the base line drawn with pencil?

Also, why does the sample of the substance have to be a SMALL DOT?

Any other notes or info on paper chromatography is also welcome.

Thanks in advance!

Top 2 Answers
A few days ago
old guy

Favorite Answer

The solvent refers to any substance capable of solubilizing other substance as ink.During the chromatographic process the analyte experiences zone broadening as a result of diffusion. Two analytes with different retention times yet with large broadening do not resolve and this is why in any chromatographic system broadening needs to be minimized. This is done by selecting the proper stationary and mobile phase, the eluent velocity, the track length and temperature.
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A few days ago
Anonymous
………….

Ask the teacher, maybe?

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